Method for detecting the presence of different antinuclear antibody fluorescence pattern types and apparatus for this purpose

US11367187B2 · kind B2 · utility

Assignee

• EUROIMMUN Medizinische Labordiagnostika AG · DE

Inventors

• Jens Krauth · Stade, DE
• Christopher Krause · Bad Honnef, DE
• Joern Voigt · Hof Perlberg, DE
• Melanie Hahn · Stockelsdorf, DE
• Christian Marzahl · Erlangen, DE
• Stefan Gerlach · Groß, DE

Key dates

• Filing date: Jul 2, 2020
• Grant date: Jun 21, 2022
• Expiry date: Sep 1, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/30024
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method is for detecting respective potential presence of respective different antinuclear antibody fluorescence pattern types on a biological cell substrate including human epithelioma cells, including: acquiring a first image which represents staining of the cell substrate by a first fluorescent dye, and acquiring a second image which represents staining of the cell substrate by a second fluorescent dye, detecting, on the basis of the first image, respective image segments which in each case represent at least one mitotic cell, selecting, on the basis of the detected image segments, subimages of the first image and subimages corresponding thereto of the second image and detecting, on the basis of the selected subimages of the first image and of the selected subimages of the second image, respective actual presence of respective cellular fluorescence pattern types by means of a convolutional neural network.