Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
US11371028B2 · kind B2 · utility
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13Claims
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Key dates
| Filing date | Jan 25, 2021 |
| Grant date | Jun 28, 2022 |
| Priority date | — |
| Expiry date | Jan 25, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Variants of the bacteriophage B103 DNA polymerase are described herein. The variant has improved properties, that include when compared to wild-type Phi29 DNA polymerase, at least one of the following: increased thermostability, improved reaction rate for DNA amplification, reduced background and a reduction of bias. Methods of using the DNA polymerase variant are also described herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.