Methods for producing lipases

US11371029B2 · kind B2 · utility

Assignee

• Novozymes A/S · DK

Inventors

• Allan Svendsen · Paseo Los Muchachos, DK
• Rakhi Saikia · Bengaluru, IN
• Sohel Dalal · Ahmedabad, IN
• Vikram Kjoeller Bhatia · Copenhagen, DK
• Lone Baunsgaard · Elsinore, DK

Key dates

• Filing date: May 4, 2020
• Grant date: Jun 28, 2022
• Expiry date: May 4, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y301/01003
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method for generating a preparation of lipase with increased lipolytic activity comprising a step of altering one or more nucleotides in a polynucleotide comprising a first polynucleotide encoding a propeptide operationally linked to a second polynucleotide encoding a lipase, wherein the alteration of one or more nucleotides are made in the first polynucleotide and the alteration independently results in a substitution, an insertion or a deletion in the encoded propeptide amino acid sequence.