Patent · US Active

Polymerase chain reaction normalization through primer titration

US11371076B2 · kind B2 · utility

0Cited by

146References

27Claims

0Family size

Assignee

Becton, Dickinson and Company · US

Inventors

Katherine Lazaruk · Redwood City, US
Eleen Shum · San Jose, US
Christina Chang · Menlo Park, US

Key dates

Filing date	Jan 15, 2020
Grant date	Jun 28, 2022
Priority date	—
Expiry date	Mar 26, 2040

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2563/185
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.