Patent · US Active

Cleavable hairpin primers

US11371082B2 · kind B2 · utility

0Cited by
4References
18Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 8, 2020
Grant dateJun 28, 2022
Priority date—
Expiry dateAug 8, 2040

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6827
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.