Method for removing and/or detecting nucleic acids having mismatched nucleotides
US11371088B2 · kind B2 · utility
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Key dates
| Filing date | Jun 11, 2018 |
| Grant date | Jun 28, 2022 |
| Priority date | — |
| Expiry date | May 7, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6874
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein, among other things, are various in vitro methods that involve cleaving dsDNA molecules that comprise a mismatched nucleotide using EndoMS. In some embodiments, the method may comprise ligating a T-tailed double-stranded adapter to A-tailed double-stranded fragments of nucleic acid to produce ligation products that comprise adapter-ligated fragments and double-stranded adapter dimers that comprise a T:T mismatch at the ligation junction and cleaving both strands of the adapter dimers using EndoMS.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.