Amylase mutant having high specific activity and thermal stability, gene of mutant, and applications thereof
US11377647B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 21, 2019 |
| Grant date | Jul 5, 2022 |
| Priority date | — |
| Expiry date | Mar 21, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01001
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the field of agriculture biotechnology, specially relates to an amylase mutant having high specific activity and thermal stability, gene and use thereof. Said amylase mutant is obtained by performing substitution of S33A/S34E/V35H, and deletion of amino acids at the sites of 178 and 179 of the wild type amylase having amino acid sequence of SEQ ID NO:1, and having improved enzymatic activity and thermal stability than the wild type amylase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.