Method for preparing vanillin by fermentation with eugenol as substrate
US11377670B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 30, 2020 |
| Grant date | Jul 5, 2022 |
| Priority date | — |
| Expiry date | Dec 30, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure discloses a method for preparing vanillin by fermentation with eugenol as a substrate, preparing vanillin by a mixed fermentation of Penicillium simplicissimum OMK-68 and Bacillus sp. OMK-69, its potency reached 35 g/L, the mass conversion rate of eugenol reaches 83.3%. Penicillium simplicissimum OMK-68 in the present disclosure can use eugenol as a substrate for fermentation to prepare coniferyl alcohol, and its fermentation effect and substrate utilization rate are far better than wild-type Penicillium simplicissimum. The Bacillus sp. OMK-69 in the present disclosure can use coniferyl alcohol as a substrate for fermentation to prepare vanillin, and its fermentation effect and substrate utilization rate are far better than wild-type Bacillus sp.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.