Methods and tools for purifying nucleic acids and using polymerized tubulin
US11384351B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 27, 2018 |
| Grant date | Jul 12, 2022 |
| Priority date | — |
| Expiry date | Nov 27, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the field of nucleic acid purification. In particular, it relates to methods and tools for purifying nucleic acids in a sample; which are compatible with high-throughput sequencing and diagnosis. The inventors have shown that nucleic acid binding proteins recruited to polymerized tubulin (i.e. microtubules) could, subsequently, be isolated from cell lysates. Surprisingly, it has now been found that the amount of recovered nucleic acid found in these microtubule pellets increases dramatically in the presence of nucleic acid-trapping proteins comprising a nucleic acid-binding moiety and a polymerized tubulin-binding moiety, by comparison to proteins devoid of the nucleic acid-binding moiety; and that the recovery of the purified nucleic acids was itself particularly efficient. This purification method is particularly amenable to high-throughput sequencing and/or in the context of a diagnosis method for identifying or comparing the amount of nucleic acids in a set of samples.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.