Method of generating an antibody naïve library, said library and application(s) thereof

US11384354B2 · kind B2 · utility

Assignee

• Zumutor Biologics Inc. · US

Inventors

• Sohang Chatterjee · Kanchinakote, IN
• Kavitha Iyer Rodrigues · Bengaluru, IN
• Maloy Ghosh · Kanchinakote, IN
• Sunit Maity · Kanchinakote, IN
• Divya Unnikrishnan · Kanchinakote, IN
• Yogendra Manjunath Bangalore Muniraju · Kanchinakote, IN
• Sathyabalan Murugesan · Bengaluru, IN
• Pavithra Mukunda · Kundapura, IN
• Bhargav Prasad · Komangalam, IN
• Veeresha Kamanagowda · Kanchinakote, IN
• Sanghamitra Bhattacharjee · Kanchinakote, IN
• Pravin Kumar Dakshinamurthy · Komangalam, IN
• Vivek Halan · Viralimalai, IN
• Sankaranarayanan Srinivasan · Kanchinakote, IN
• Anuradha Hora · Sitapur, IN
• Bairavabalakumar Natarajan · Chennai, IN
• Karthika Nair · Survanagiri, IN
• Aswini Thanigaivel · Chennai, IN
• Amol Maliwalave · Survanagiri, IN
• Bharath Ravindra Shenoy · Kanchinakote, IN
• Sahana Bhima Rao · Kanchinakote, IN
• Subhra Prakash Chakrabarty · Survanagiri, IN
• Ashvini Kumar Dubey · Kanchinakote, IN
• Amir Khan · Aligarh, IN
• Anurag TIWARI · Sidhauli, IN
• Santosh Kumar · Burmu, IN
• Shivani Patel · Damoh, IN
• Nikitha M · Survanagiri, IN

Key dates

• Filing date: Dec 21, 2015
• Grant date: Jul 12, 2022
• Expiry date: Jul 11, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B50/06
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present disclosure relates to a method of generating an antibody library, not limiting to a human naïve antibody gene expression library encompassing a pool of nucleic acid sequences derived from a natural antibody repertoire comprising humoral immunity from healthy and genetically diverse human populations. More specifically, the method employs a combination of phage and/or yeast antibody surface display concept which allows to screen large antibody library size and facilitates better folding of antibody structure. The present disclosure also relates to a human naïve antibody library generated by employing the process of the present disclosure, a set of primers employed in the method and application(s) of said antibody library.