Patent · US Active

In vitro isolation and enrichment of nucleic acids using site-specific nucleases

US11384383B2 · kind B2 · utility

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1References
20Claims
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Assignee

Inventors

Key dates

Filing dateAug 8, 2018
Grant dateJul 12, 2022
Priority date—
Expiry dateFeb 5, 2039

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2521/301
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention relates to a method for the isolation of a target nucleic acid region. In particular, said method comprises the steps of contacting a population of nucleic acid molecules with at least one Type II Cas protein-gRNA complex, wherein said gRNA comprises a guide segment that is complementary to the sequence comprised in the target region of at least one nucleic acid molecule, thereby forming a Type II Cas protein-gRNA-nucleic acid complex, contacting the population of nucleic acid molecules with at least one enzyme having exonuclease activity, and isolating the target nucleic acid region from the Type II Cas protein-gRNA-nucleic acid complex.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.