Optical method for estimating a representative volume of particles present in a sample based on a size of each region of interest
US11385158B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 30, 2018 |
| Grant date | Jul 12, 2022 |
| Priority date | — |
| Expiry date | Mar 21, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30024
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention is a method for estimating a representative volume of particles of interest (10 i) immersed in a sample, the sample extending in at least one plane, referred to as the sample plane (P 10), the sample comprising a sphering agent, capable of modifying the shape of the particles, the method comprising the following steps: a) illuminating the sample by means of a light source (11), the light source emitting an incident light wave (12) propagating towards the sample (10) along a propagation axis (Z); b) acquiring, by means of an image sensor (16), an image (I 0) of the sample (10), formed in a detection plane (P 0), the sample being arranged between the light source (11) and the image sensor (16), each image being representative of a light wave (14) referred to as an exposure light wave, to which the image sensor (16) is exposed under the effect of illumination; c) using the image of the sample (I 0), acquired during step b), and a holographic propagation operator, to calculate a complex expression (A (x, y, z)) of the exposure light wave (14) in different positions relative to the detection plane; the method comprising a step of estimating the representative volume (AA) o…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.