Patent · US Active

Amplification technology using dual enzyme cascade detection

US11385229B2 · kind B2 · utility

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1Claims

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Assignee

The Regents of the University of California · US

Inventors

Niren Murthy · Atlanta, US
Lee W. Riley · New York, US
Tara Renee BeBoer · Berkeley, US

Key dates

Filing date	Aug 14, 2019
Grant date	Jul 12, 2022
Priority date	—
Expiry date	Sep 3, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/34
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Diagnostic assays and methods employ a dual-enzyme cascade system comprised of two enzymatic amplifiers and two molecular probes, by (a) incubating a target enzyme with a target enzyme substrate to liberate a thiol; (b) incubating the thiol with a disulfide inactivated amplification enzyme to activate the amplification enzyme in an interchange reaction of the thiol and the disulfide; and (c) incubating the activated amplification enzyme with an amplification enzyme substrate to generate an amplified signal.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.