Process for generation, identification and isolation of human pluripotent stem cell-derived cardiomyocytes and cardiomyocyte subpopulations

US11390851B2 · kind B2 · utility

Assignee

• MILTENYI BIOTEC B.V. & CO. KG · DE

Inventors

• Kristin Becker · Moitzfeld, DE
• Dominik Eckardt · Moitzfeld, DE
• Andreas Bosio · Cologne, DE

Key dates

• Filing date: May 30, 2017
• Grant date: Jul 19, 2022
• Expiry date: May 23, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2506/45
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a method for generation, isolation, detection and/or analysis of cardiomyocytes derived from a starting cell composition comprising pluripotent and/or multipotent stem cells, the method comprising the steps a) differentiating said pluripotent and/or multipotent stem cells into cardiovascular cells, thereby generating a sample comprising cardiomyocytes and non-cardiomyocytes; and b) labeling the non-cardiomyocytes of said sample with more than one antibody or antigen binding fragment thereof specific for antigens of non-cardiomyocytes and c) depleting said labeled non-cardiomyocytes from said sample; and optionally d) labeling the cardiomyocytes of said sample with at least one antibody or antigen binding fragment thereof specific for antigen(s) of cardiomyocytes; and e) enriching said labeled cardiomyocytes and detecting and isolating cardiomyocyte subtypes derived from said pluripotent and/or multipotent stem cells.