Increasing protein yield in plants
US11390878B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 26, 2015 |
| Grant date | Jul 19, 2022 |
| Priority date | — |
| Expiry date | May 2, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/60
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of increasing the yield, stability, or both of an acid sensitive protein in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, or portion of the plant. The first nucleic acid comprises a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding the acid sensitive protein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a channel protein, for example but not limited to a proton channel protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby increasing the yield of the acid sensitive protein when compared to the yield of the acid sensitive protein produced in the plant or portion of the plant produced under the same conditions, and in the absence of the proton channel protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.