Methods and compositions for discrete melt analysis
US11390902B2 · kind B2 · utility
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Key dates
| Filing date | Jan 22, 2019 |
| Grant date | Jul 19, 2022 |
| Priority date | — |
| Expiry date | Aug 16, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2531/113
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and reagent for determining the presence and/or for quantifying the amount of a target nucleic acid sequences in a sample are provided. In some aspects, the methods comprise performing a melt analysis by detecting, a signal from a probe at a temperature that is lower than the Tm of the probe and a signal at a temperature that is higher than the Tm of the probe, without detecting a signal at the Tm of the probe.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.