Liquid chromatography/mass spectrometry methods for the analysis of polar molecules
US11391707B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 25, 2019 |
| Grant date | Jul 19, 2022 |
| Priority date | — |
| Expiry date | Jan 24, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/884
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A mixed-mode chromatography method for the determination of phosphorylated sugars in a sample is provided. The mixed-mode chromatography method includes obtaining a sample comprising at least one phosphorylated sugar. The sample is introduced onto a chromatography system. The chromatography system includes a column having a stationary phase material contained inside the column. The stationary phase material has a surface comprising a hydrophobic surface group and at least one ionizable modifier. The sample with a mobile phase eluent is flowed through the column, where the at least one phosphorylated sugar is substantially resolved and retained within seven minutes. The mobile phase eluent includes water with an additive and acetonitrile with the additive. The mobile phase eluent has a pH less than 6. The at least one phosphorylated sugar is detected using a detector.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.