Particle-based immunoassay using a pegylated analyte-specific binding agent

US11391733B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Toralf Zarnt · Penzberg, DE
• Dieter Heindl · Munich, DE
• Alfons Nichtl · Hohenpeißenberg, DE
• Frank Sicherl-Birk · Penzberg, DE

Key dates

• Filing date: Feb 19, 2018
• Grant date: Jul 19, 2022
• Expiry date: Feb 19, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54393
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed is a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein the microparticles are coated with the first partner of a binding pair, the method involving mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of containing or containing the analyte, wherein the second partner of the binding pair is bound to the analyte-specific binding agent via a linker having from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.