Patent · US Active

Composition and methods for detecting adenosine modifications

US11396672B2 · kind B2 · utility

0Cited by

0References

16Claims

0Family size

Assignee

THE UNIVERSITY OF CHICAGO · US

Inventors

Chuan He · Chicago, US
Kai Chen · Rockville, US
Qing Dai · San Jose, US

Key dates

Filing date	Aug 30, 2016
Grant date	Jul 26, 2022
Priority date	—
Expiry date	Mar 22, 2038

Classification

Technology area (CPC G)Physics
CPC primaryG01N2333/978
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The current disclosure relates to methods, compositions and kits for detecting modified adenosine in a target RNA molecule. Aspects relate to a method for detecting modified adenosine in a target ribonucleic acid (RNA) comprising contacting the target RNA with an adenosine deaminase enzyme (adenosine deaminase, RNA-specific) to generate a target RNA with deaminated adenosines and sequencing the target RNA with deaminated adenosines; wherein the modified adenosine is detected when the nucleotide sequence is adenosine.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.