Single-pass primary analysis
US11397870B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 10, 2021 |
| Grant date | Jul 26, 2022 |
| Priority date | — |
| Expiry date | Dec 10, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06V2201/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and systems for image analysis are provided, and in particular for identifying a set of base-calling locations in a flow cell for DNA sequencing. These include capturing flow cell images after each sequencing step performed on the flow cell, and identifying candidate cluster centers in at least one of the flow cell images. Intensities are determined for each candidate cluster center in a set of flow cell images. Purities are determined for each candidate cluster center based on the intensities. Each candidate cluster center with a purity greater than the purity of the surrounding candidate cluster centers within a distance threshold is added to a template set of base-calling locations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.