System, method, and composition for incubating spores for use in aquaculture, agriculture, wastewater, and environmental remediation applications
US11401500B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 23, 2019 |
| Grant date | Aug 2, 2022 |
| Priority date | — |
| Expiry date | Aug 6, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/60
- WIPO fieldEnvironmental technology
- WIPO sectorChemistry
Abstract
A system and method for generating an incubated bacteria solution by heating a nutrient germinant composition and bacteria, including at least one species in spore form, to a preferred temperature a range of 35-50° C. for 2-60 minutes using exothermic chemical reaction heat. An incubated bacteria solution is preferably generated at or near a point-of-use in an aquaculture, agriculture, wastewater, or environmental remediation application. The nutrient-germinant composition comprises L-amino acids, optionally D-glucose and/or D-fructose, a buffer, an industrial preservative, and may include bacteria spores (preferably of one or more Bacillus species) or they may be separately combined for incubation. A first chemical contained in a pouch is activated by contact with a second chemical, water, or air in a flameless heater to initiate exothermic reaction to provide incubation heat. A potable, single-use incubation bag is configured to hold the flameless heater and a container of nutrient germinant composition and spores.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.