Methods of producing promoter variants

US11401523B2 · kind B2 · utility

Assignee

• LONZA LTD · CH

Inventors

• Diethard Mattanovich · Wien, AT
• Brigitte Gasser · Wien, AT
• Michael Maurer · Wien, AT
• Roland Prielhofer · Wien, AT
• Joachim Klein · Hochspeyer, DE
• Jana Wenger · Visp, CH

Key dates

• Filing date: Mar 5, 2019
• Grant date: Aug 2, 2022
• Expiry date: Aug 13, 2040

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.