Selective fluorescent probe for aldehyde dehydrogenase

US11407725B2 · kind B2 · utility

Assignee

• The Board of Trustees of the University of Illinois · US

Inventors

• Thomas Edward Bearrood · Urbana, US
• Chelsea Diane Anorma · Urbana, US
• Jefferson Chan · Berkeley, US

Key dates

• Filing date: Dec 18, 2019
• Grant date: Aug 9, 2022
• Expiry date: Oct 22, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/573
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

High aldehyde dehydrogenase 1A1 (ALDH1A1) activity has emerged as a reliable marker for the identification of both normal and cancer stem cells. Herein, is presented AlDeSense, a turn-on green fluorescent probe for aldehyde dehydrogenase 1A1 (ALDH1A1) and Ctrl-AlDeSense, a matching non-responsive reagent. AlDeSense exhibits a 20-fold fluorescent enhancement when treated with ALDH1A1. Through the application of surface marker antibody staining, tumorsphere assays, and assessment of tumorigenicity, the disclosed results show that cells exhibiting high AlDeSense signal intensity have properties of cancer stem cells. Herein, is also reported the development of a red congener, red-AlDeSense. Importantly, red-AlDeSense represents one of only a few examples of a turn-on sensor in the red region using the d-PeT quenching mechanism.