Method for preparation and screening of fungal mutant with high hydrolytic activity and catabolite derepressed character

US11407974B2 · kind B2 · utility

Assignees

• Indian Oil Corporation Limited · IN
• Department of Biotechnology · IN

Inventors

• Mukund Adsul · Faridabad, IN
• Simranjeet Kaur Sandhu · Faridabad, IN
• Reeta Rani Singhania · Faridabad, IN
• Jitendra Kumar Saini · Faridabad, IN
• Anshu Shankar MATHUR · Faridabad, IN
• Ravi Prakash GUPTA · Sindhar, IN
• Deepak Kumar Tuli · Faridabad, IN
• Suresh Kumar Puri · Faridabad, IN
• Sankara Sri Venkata RAMAKUMAR · Faridabad, IN

Key dates

• Filing date: Sep 26, 2019
• Grant date: Aug 9, 2022
• Expiry date: Feb 29, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y302/01055
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a mutant fungal strain of Penicillium funiculosum ‘MRJ-16’ characterized by the ability to produce high titer of enzyme mixture comprising FPase, CMCase, Cellobiase, β-glucosidase, endoglucanase, α-L arabinofuranosidase, β-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases and produce enzymes in the presence of a catabolite repressor molecule like glucose and/or xylose. The titer of enzyme mixture produced using mutant fungal strain MRJ-16 is at least two fold higher than naive Penicillium funiculosum strain NCIM 1228, when used in a fermentation process. The mutant strain ‘MRJ-16’ with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.