Methods for selectively suppressing non-target sequences

US11408024B2 · kind B2 · utility

Assignee

• Molecular Loop Biosciences, Inc. · US

Inventors

• Jeff Gole · Cambridge, US
• Athurva Gore · Cambridge, US
• Mark Umbarger · Brookline, US

Key dates

• Filing date: Sep 9, 2015
• Grant date: Aug 9, 2022
• Expiry date: Sep 9, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention generally relates to negative selection of nucleic acids. The invention provides methods and systems that remove unwanted segments of nucleic acid in a sample so that a target gene or region of interest may be analyzed without interference from the unwanted segments. A sample is obtained that includes single-stranded nucleic acid with one or more unwanted segments. Complementary nucleic acid is added to the single-stranded nucleic acid to create a double-stranded region that includes the unwanted segment. The double-stranded region is then digested, leaving single-stranded nucleic acid that includes the target gene or region of interest. This allows paralogs, pseudogenes, repetitive elements, and other segments of the genome that may be similar to the target gene or region of interest to be removed from the sample.