Stable nanoscale nucleic acid assemblies and methods thereof

US11410746B2 · kind B2 · utility

Assignee

• Massachusetts Institute of Technology · US

Inventors

• Remi Veneziano · Manassas, US
• Sakul Ratanalert · Cambridge, US
• Tyson Shepherd · Louisville, US
• Hyungmin Jun · Arlington, US
• Mark Bathe · Cambridge, US

Key dates

• Filing date: Apr 27, 2017
• Grant date: Aug 9, 2022
• Expiry date: Jul 15, 2038

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A90/10
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods for the top-down design of nucleic acid nanostructures of arbitrary geometry based on target shape of spherical or non-spherical topology are described. The methods facilitate 3D molecular programming of lipids, proteins, sugars, and RNAs based on a DNA scaffold of arbitrary 2D or 3D shape. Geometric objects are rendered as node-edge networks of parallel nucleic acid duplexes, and a nucleic acid scaffold routed throughout the network using a spanning tree formula. Nucleic acid nanostructures produced according to top-down design methods are also described. In some embodiments, the nanostructures include single-stranded nucleic acid scaffold, DX crossovers, and staple strands. In other embodiments, the nanostructures include single-stranded nucleic acid scaffold, PX crossovers and no staples. Modified nanostructures include chemically modified nucleotides and conjugated to other molecules are described.