Methods for detecting and determining levels of monolignol ester conjugates incorporated into lignin and compounds relating thereto
US11414372B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 2019 |
| Grant date | Aug 16, 2022 |
| Priority date | — |
| Expiry date | Jul 28, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2560/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods of detecting and, optionally, determining a level of incorporation of monolignol ester conjugates into lignin. The methods include derivatizing lignin to acylate at least a portion of free phenolic and aliphatic hydroxyls and to halogenate at least a portion of benzylic alcohols present in the lignin to yield derivatized lignin, treating the derivatized lignin with a reducing agent to cleave at least a portion of the derivatized lignin to yield lignin cleavage products, acetylating at least a portion of free hydroxyl groups in the lignin cleavage products with a labeled acetylation agent to yield labeled lignin fragments, and detecting the labeled lignin fragments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.