Construction method of Mucor circinelloides cell factory for producing dihomo-gamma-linolenic acid and fermentation technology
US11414650B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 12, 2020 |
| Grant date | Aug 16, 2022 |
| Priority date | — |
| Expiry date | Dec 12, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/785
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to a construction method of a Mucor circinelloides cell factory for producing dihomo-γ-linolenic acid and a fermentation technology, belonging to the field of genetic engineering. In the present disclosure, γ-linolenic acid elongase gene glelo is obtained from Mortierella alpine by cloning, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and the gene glelo is integrated into Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-glelo, and finally, the expression of the gene glelo in Mucor circinelloides is realized. The lipid content in the recombinant strain Mc-glelo is not obviously different from that in the control strain Mc1552, however, the lipid composition changes greatly, and dihomo-γ-linolenic acid appears in the lipids of the recombinant strain Mc-glelo, and the content thereof reaches 5.7% of the total fatty acids. Under optimized fermentation conditions and in the presence of precursor fatty acid, the DGLA content reaches 7.6%. The new recombinant strain was deposited in China General Microbiological Culture Collection C…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.