Promoter useful for high expression of a heterologous gene of interest in Aspergillus niger

US11414653B2 · kind B2 · utility

Assignees

• National Technology & Engineering Solutions of Sandia, LLC · US
• Battelle Memorial Institute · CH
• The Regents of the University of California · US

Inventors

• John M. Gladden · Alameda, US
• Saori Amaike Campen · Madison, US
• Jinxiang Zhang · Albany, US
• Jon K. Magnuson · Richland, US
• Scott E. Baker · San Jose, US
• Blake A. Simmons · San Francisco, US

Key dates

• Filing date: Sep 6, 2018
• Grant date: Aug 16, 2022
• Expiry date: Jan 27, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y302/01021
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.