Method for inserting desired DNA fragment into site located adjacent to binding domain of DNA-binding protein
US11414680B2 · kind B2 · utility
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8Claims
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Key dates
| Filing date | May 24, 2017 |
| Grant date | Aug 16, 2022 |
| Priority date | — |
| Expiry date | Jan 28, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1093
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for integrating a DNA fragment of a desired base sequence into a site located adjacent to a binding region of a DNA-binding protein bound to a DNA molecule, the method including bringing the DNA fragment having a base sequence including a transposase-binding sequence and the desired base sequence close to the binding region using a specific binding substance to the DNA-binding protein, binding transposase to the transposase-binding sequence, and activating the transposase such that the DNA fragment of the desired base sequence is integrated into the site located adjacent to the binding region.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.