Thiol-containing cleave reagents and oxidative wash
US11421264B2 · kind B2 · utility
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32Claims
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Key dates
| Filing date | Mar 13, 2017 |
| Grant date | Aug 23, 2022 |
| Priority date | — |
| Expiry date | Mar 13, 2037 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.