In situ readout of DNA barcodes

US11421273B2 · kind B2 · utility

Assignee

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• Amjad Askary · Pasadena, US
• Michael B. Elowitz · Los Angeles, US
• Mark W. Budde · Arcadia, US
• Carlos Lois · South Pasadena, US
• Luis Sanchez Guardado · Pasadena, US
• Long CAI · Port Jefferson Station, US
• James Linton · Pasadena, US

Key dates

• Filing date: Dec 2, 2019
• Grant date: Aug 23, 2022
• Expiry date: Jun 12, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N1/30
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed herein include systems, methods, compositions, and kits for in situ readout of barcodes, such as DNA barcodes. Barcode constructs containing a promoter (e.g., a phage promoter) that is inactive in live cells can be integrated in the genomes of cells. Cells can be fixed, and phage RNA polymerase can be used for transcription of the barcode to RNA transcripts. The RNA transcripts can be detected using, for example, fluorescent imaging and used to determine barcode sequences.