Method and device to optimize analyte and antibody substrate binding by least energy adsorption
US11422129B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 20, 2005 |
| Grant date | Aug 23, 2022 |
| Priority date | — |
| Expiry date | Dec 4, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54366
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method of making an assay device for conducting an assay to detect a concentration of an analyte in a sample fluid. The assay devices would typically have a substantially planar surface having a series of site specific immobilized calibration spot arrays containing pre-determined quantities of the analyte printed thereon. In addition, a series of site specific immobilized test spot arrays, including capture antibody for binding the analyte protein is printed on the assay device. The method involves first modifying the planar surface to provide hydrophobic binding sites, hydrophilic linking and covalent bonding sites. Then the method requires printing the series of site specific immobilized test spot arrays and the series of site specific immobilized calibration spot arrays on the substantially planar surface. Applying the sample fluid to the assay device is the next step followed by testing a sensitivity of the assay and modulating ratios of the hydrophobic, hydrophilic and covalent binding sites in order to optimize the sensitivity of the assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.