Time-resolved imaging method with high spatial resolution
US11422348B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 24, 2019 |
| Grant date | Aug 23, 2022 |
| Priority date | — |
| Expiry date | Jun 22, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B27/58
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
A method for operating a point laser-scanning microscope includes scanning a sample with a focused illumination laser beam; recording a plurality of images by detecting elements being configurable to an intensity mode, in which the recorded images are intensity images gi,j(n) related to photons collected during an entire dwell time of the illumination beam on an individual position n, or to a time-resolved mode, in which the recorded images are time-resolved images gi,jt(n, t), the collected photons being discriminated based on their arrival times to individual detecting elements; calculating a fingerprint image a by summing the plurality of intensity images gi,j(n) over all positions n; estimating shift matrices sx and sy from the intensity images gi,j(n); reconstructing at least one of a time-resolved object function ft and an intensity object function f; and visualizing at least one of a high-resolution time-resolved image ft˜ and a high-resolution intensity image f˜.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.