Continuous sample purification systems and methods
US11426679B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 21, 2016 |
| Grant date | Aug 30, 2022 |
| Priority date | — |
| Expiry date | Nov 7, 2038 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D15/3847
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Sample purification systems include a particle extraction assembly having a mixing compartment and a settling compartment. A biological sample is mixed with two liquid phases formulated to effectuate transfer of a biological molecule into a first phase and particulate contaminants into a second phase. The first phase includes a solubilizing salt, the second phase includes an organic molecule, and the mixture can have little or no monoatomic salt or dextran. The molecule-containing first phase can be optionally concentrated without also concentrating the particulate contaminants and introduced into a multi-stage liquid-liquid extractor, by which the biological molecule or molecular contaminants are extracted from the first phase into a third phase, thereby purifying the molecule away from contaminants. The extracted sample can be further purified through a series of processing steps. The system can be run in continuously mode to maintain sterility of the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.