Scatter labeled imaging of microvasculature in excised tissue (SLIME)
US11427801B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 9, 2018 |
| Grant date | Aug 30, 2022 |
| Priority date | — |
| Expiry date | May 14, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30024
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure relates to a simple, fast, and low cost method for 3D microvascular imaging, termed “scatter labeled imaging of microvasculature in excised tissue” (SLIME). The method can include perfusing a contrast agent through vasculature of a tissue sample with a contrast perfusing unit (22). The contrast agent can include colloids and a dispersant. After the contrast agent is perfused through the vasculature, the vasculature of the tissue sample can be treated with a cross-linking agent delivery unit (24) providing a molecule that cross links with at least a portion of the dispersant to form a sticky, non-Newtonian polymer that prevents leakage of the contrast agent out of the vasculature of the tissue sample. The tissue sample can then be immersed in a solution comprising a clearing agent with an optical clearing unit (26) and subsequently imaged.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.