Aspergillus niger seed continuous culture and method for producing citric acid therefrom
US11434467B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 24, 2018 |
| Grant date | Sep 6, 2022 |
| Priority date | — |
| Expiry date | Dec 1, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/685
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is an Aspergillus niger seed continuous culture method, comprising the steps of: (1) at a startup stage, Aspergillus niger spores are inoculated into a seed culture medium to obtain a seed liquid; (2) at a seed continuous culture stage, continuous dispersion treatment is performed on the seed liquid obtained in step (1), continuous culture is performed on the seed liquid obtained by dispersion, and meanwhile, a fresh seed feed medium is replenished; and (3) at a stop stage, the replenishment of the fresh seed feed medium and the dispersion treatment are stopped, continuous culture is performed to obtain a seed liquid, and then the seed liquid is transferred into the fermentation medium for fermentation culture. The method according to the present invention makes breakthrough to solve problems that multi-cellular filamentous bacteria grow slowly and mycelium pellets are easy to lose in continuous culture, thus fully achieving seed continuous culture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.