Glucose oxidase GOD mutant and gene and application thereof
US11434473B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Dec 20, 2018 |
| Grant date | Sep 6, 2022 |
| Priority date | — |
| Expiry date | Dec 20, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y101/03004
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention takes the glucose oxidase GOD from Aspergillus niger as the mutation template to obtain the glucose oxidase GOD mutants with improved catalytic efficiency and thermal stability by site directed mutagenesis. The specific activity of the mutant of the present invention is 66% higher than that of the wild type GOD; the enzyme activity of the mutant of the present invention is 13.6 times higher than that of the wild type after being treated at 70° C. for 10 min; and the enzyme activity of the mutant of the present invention is 29.4 times higher than that of the wild type after being treated at 80° C. for 2 min.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.