Method for constructing single cell sequencing library and use thereof

US11434483B2 · kind B2 · utility

Assignee

• MGI Tech Co., Ltd. · CN

Inventors

• Longqi LIU · Lo Wu, CN
• Chuanyu Liu · Longbeilingcun, CN
• Liang Wu · Auburn, US
• Zhouchun Shang · Longbeilingcun, CN
• Mengnan Cheng · Longbeilingcun, CN
• Yue Yuan · Longbeilingcun, CN
• Liqin Xu · Longbeilingcun, CN
• Xin Liu · Dongguan, CN
• Xun Xu · Shanghai, CN

Key dates

• Filing date: Dec 7, 2016
• Grant date: Sep 6, 2022
• Expiry date: Apr 18, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided in the present invention is a method for constructing single cell sequencing libraries, comprising the following steps: a) lysing a single cell to obtain a single cell lysate; b) separating the nucleus and the cytoplasm in the single cell lysate obtained in step a) to obtain a nuclear solution and a total RNA solution; and c) constructing a chromatin DNA library with the nuclear solution obtained in step b) to obtain a chromatin-accessibility sequencing library of the single cell; and constructing a transcriptome library with the total RNA solution obtained in step b) to obtain a transcriptome sequencing library of the single cell.