Patent · US Active

Method for detecting selenoamino acid in selenoprotein

US11435325B2 · kind B2 · utility

0Cited by

1References

9Claims

0Family size

Assignee

ENSHI TUJIA AND MEAO AUTONOMOUS PREFECTURE ACADEMY OF AGRILCULTURAL SCIENCE (ENSHI TUJIA AND MIAO AUTONOMOUS PREFECTURE SELENIUM APPLICATION TECHNOLOGY AND PRODUCT DEVELOPMENT INSTITUTE) · CN

Inventors

Yongbo Chen · Foshan, CN
Shuqin Liu · Hubei, CN
Chaoyang Zhang · Hunan, CN
Baishun Hu · Hubei, CN
Weidong Li · Cupertino, US
Guangyu Huang · Hubei, CN
E Chen · Lo Wu, CN
Bang Qin · Hubei, CN
Yong Qu · Hubei, CN

Key dates

Filing date	Aug 20, 2020
Grant date	Sep 6, 2022
Priority date	—
Expiry date	Apr 29, 2041

Classification

Technology area (CPC G)Physics
CPC primaryG01N2030/8831
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present invention belongs to the technical field of analysis and detection and provides a method for detecting a selenoamino acid in a selenoprotein. The detection method includes: (1): mixing 10-30 mg of a selenoprotein sample with hydrochloric acid, and hydrolyzing with microwaves at 140-170° C. for 10-40 min to obtain a hydrolysate; (2): adjusting pH of the hydrolysate to 6-8 to obtain a pretreated solution; and, (3): detecting a selenoamino acid in the pretreated solution with high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) to obtain a content of the selenoamino acid. The method is simple in operation and provides an accurate and reliable result, and also reduces time and cost.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.