System and method for intensity stabilization for quantitative imaging
US11442018B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 23, 2019 |
| Grant date | Sep 13, 2022 |
| Priority date | — |
| Expiry date | Jan 23, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6452
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A system (90) for imaging microscopic samples comprises a light source (31) for exciting fluorescence from at least one sample, a photosensor (40) configured to detect light deflected by a beam splitter (20) from an optical excitation path directed to the sample and output an electrical signal of optical flux to the sample, a camera (30) configured to receive and form an image of fluorescence light emitted from the sample, and a controller (134) comprising an integrator (122) configured to integrate the electrical signal from the photodetector and a comparator configured to compare the integrated output to a predetermined threshold, wherein the controller is configured to control an exposure time of the camera such that each sample receives substantially the same total optical flux of incident light during a duration of camera exposure which is terminated when a predetermined threshold representative of the total optical flux is met.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.