Method for identifying drug-discovery target protein for development of antibody drug, and method for producing antibody against target protein
US11442066B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 19, 2018 |
| Grant date | Sep 13, 2022 |
| Priority date | — |
| Expiry date | Oct 15, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54353
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Finding a protein of a minute amount present on a cell membrane to provide a method for producing an antibody against the protein. Producing an antibody using a protein identified by an identification method including: a labeling step of using a labeling agent comprising at least one selected from bis-iminobiotin compounds and bis-biotin compounds to obtain cells having a labeled protein; a degradation step of preparing a degradation product for an immobilization treatment, the degradation product containing the labeled protein; an immobilization step of immobilizing the labeled protein contained in the degradation product for an immobilization treatment on a stationary phase via a streptavidin mutant; a cleavage step of releasing an analysis sample from the stationary phase on which the labeled protein is immobilized; and an analysis step of analyzing the analysis sample to identify the labeled protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.