Pichia pastoris yeast cultures comprising reduced antibody-associated variants

US11447540B2 · kind B2 · utility

Assignee

• H. LUNDBECK A/S · DK

Inventors

• Gary Lesnicki · Woodinville, US
• Patricia Dianne McNeill · Federal Way, US
• Franz Hartner · Kundl, AT
• Mark Young · Boulder, US

Key dates

• Filing date: Oct 24, 2018
• Grant date: Sep 20, 2022
• Expiry date: Jul 21, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/565
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.