Apparatus for and methods of the disruption of a biological cell
US11447767B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 15, 2011 |
| Grant date | Sep 20, 2022 |
| Priority date | — |
| Expiry date | Sep 26, 2032 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/049
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disrupting a biological cell includes freezing, boiling or perhaps alternately freezing and boiling material containing the biological cell using a thermoelectric cell with a working face, and a base face whereof is contiguous with a heat source/sink at a substantially constant temperature. Apparatus for the disruption process includes a peltier cell, a base face, which is flexibly attached to a heat source/sink held at a constant temperature, and a working face contiguous with a reaction vessel or holder thereof. Reversal of the voltage in the peltier cell enables the working face alternately to reach below freezing and above boiling temperatures, and/or with use of a resistive wire on the vessel or holder for heating, with the TEC used purely for cooling. The materials of the base face tend to inhibit disintegration of the peltier cell brought about by expansion and contraction by heat.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.