Flow cell with selective deposition or activation of nucleotides

US11453003B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Ali Agah · San Diego, US
• Aathavan Karunakaran · Berkeley, US
• Tarun Khurana · San Diego, US
• Stanley Hong · Palo Alto, US
• Merek Siu · Alameda, US
• Arvin Emadi · San Jose, US
• Craig Ciesla · Mountain View, US

Key dates

• Filing date: Dec 14, 2021
• Grant date: Sep 27, 2022
• Expiry date: Dec 14, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

An apparatus includes a flow cell body, a plurality of electrodes, an integrated circuit, and an imaging assembly. The flow cell body defines one or more flow channels and a plurality of wells. Each flow channel is configured to receive a flow of fluid. Each well is fluidically coupled with the corresponding flow channel. Each well is configured to contain at least one polynucleotide. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are operable to effect writing of polynucleotides in the corresponding wells. The integrated circuit is operable to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells to thereby generate polynucleotides representing machine-written data in the wells. The imaging assembly is operable to capture images indicative of one or more nucleotides in a polynucleotide.