RNA-based delivery systems with levels of control

US11453893B2 · kind B2 · utility

Assignee

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• Xiaojing Gao · Pasadena, US
• Lucy S. Chong · Pasadena, US
• Michael B. Elowitz · Los Angeles, US
• Matthew Kim · San Francisco, US

Key dates

• Filing date: Aug 29, 2019
• Grant date: Sep 27, 2022
• Expiry date: Apr 15, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2820/007
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein include methods, compositions, and systems suitable for use in delivering a polynucleotide to a target cell of a subject in need thereof. In some embodiments, a viral vector comprises a polynucleotide encoding nucleoprotein (N), phosphoprotein (P), matrix protein (M), RNA-dependent RNA polymerase (L), and one or more transgenes. The viral vector can comprise one or more of a conditionally stable fusion protein, a protease fusion protein, a degron fusion protein, and/or a glycoprotein derived of another species than the viral vector polynucleotide to enable control of viral vector transduction and/or replication.