Hybridoma cell strain and monoclonal antibody produced therefrom against serine protease of Trichinella spiralis in intestinal stage and application thereof

US11459402B2 · kind B2 · utility

Assignee

• JILIN UNIVERSITY · CN

Inventors

• Mingyuan Liu · Changchun, CN
• Xiaolei Liu · Los Angeles, US
• Yong Yang · 红钢城街道, CN
• Xuelin Wang · Indianapolis, US
• Xue Bai · Novi, US
• Bin Tang · Zhejiang, CN
• Jing Ding · Changchun, CN

Key dates

• Filing date: Dec 4, 2020
• Grant date: Oct 4, 2022
• Expiry date: Mar 26, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/26
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A hybridoma cell stain and a monoclonal antibody secreted therefrom and application thereof belong to the technical field of prevention and treatment of Trichinella spiralis (T. spiralis). Aiming at the technical problem of how to specifically diagnose trichinellosis, the disclosure provides a hybridoma cell strain deposited under an accession number of CGMCC No. 18317. Tests show that the monoclonal antibody Ts-ZH68-2A4-Ab secreted by the hybridoma cell strain can compete with the positive serum of pigs infected with T. spiralis for binding to Ts-ZH68 antigen, and the recognition peptide is 222GVDRSATCQGDSGGP236. The monoclonal antibody of the disclosure and the Ts-ZH68 protein B cell epitope polypeptide recognized by the monoclonal antibody can be used to prepare a reagent or a vaccine for diagnosing or preventing infection of T. spiralis, laying the foundation for establishment of a serological diagnosis method of T. spiralis.