Measurement of protein expression using reagents with barcoded oligonucleotide sequences

US11460468B2 · kind B2 · utility

Assignee

• Becton, Dickinson and Company · US

Inventors

• Christina Fan · San Jose, US
• Christina Chang · Menlo Park, US
• Eleen Shum · San Jose, US
• Olaf Zoellner · Leverkusen, DE
• Nidhanjali Bansal · Menlo Park, US
• James Ghadiali · San Diego, US
• Gretchen Yinbon Lam · Menlo Park, US
• Eric Jensen · Franklin Lakes, US
• Jurg Rohrer · Franklin Lakes, US
• Brent S. Gaylord · Lompoc, US
• Jody Martin · Menlo Park, US
• Jason G. Vidal · Oceanside, US

Key dates

• Filing date: Sep 25, 2017
• Grant date: Oct 4, 2022
• Expiry date: Oct 6, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G16H20/13
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.