Nucleic acid quantification using concentration-specific barcodes

US11466313B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Julia Salzman · Palo Alto, US
• Peter Wang · Oak Ridge, US
• Caroline Horn · Los Altos, US

Key dates

• Filing date: Apr 12, 2019
• Grant date: Oct 11, 2022
• Expiry date: Apr 12, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6834
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This disclosure provides, among other things, a reagent system for nucleic acid analysis. In some embodiments, the system may comprise a plurality of oligonucleotide sets each set comprising at least (a) a competitor oligonucleotide that hybridizes to a target sequence and varies in concentration from mixture to mixture and (b) a detector oligonucleotide that also hybridizes to the target sequence and contains a barcode that indicates the concentration of the competitor oligonucleotide in the oligonucleotide set. The reagent system may be used to analyze a nucleic acid sample.