Marker peptide of snake venom thrombin-like enzymes (SVTLEs) from agkistrodon halys pallas and application thereof
US11467141B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 29, 2021 |
| Grant date | Oct 11, 2022 |
| Priority date | — |
| Expiry date | Jul 29, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/8831
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to the field of chemical analysis detection and application, in particular to a marker peptide of snake venom thrombin-like enzymes (SVTLEs) from Agkistrodon Halys Pallas and an application thereof. The amino acid sequence of the marker peptide of snake venom thrombin-like enzymes (SVTLEs) from Agkistrodon Halys Pallas is TLCAGVMEGGIDTCNR. Characterizing the source of species and a content of the SVTLEs in a to-be-detected sample by using the marker peptide includes the following steps of: pretreating the to-be-detected sample by trypsin through enzymolysis, and taking a supernatant of an enzymolysis liquid as a test solution; and injecting the test solution and a reference solution into a liquid chromatography-mass spectrometer, and selecting a qualitative ion pair and a quantitative ion pair for detecting the source of species and a content of the SVTLEs in the to-be-detected sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.